Comparison of phenotypic properties of innate lymphoid cells at various stages of rheumatoid arthritis

Autoimmune diseases currently take a leading place in terms of frequency occurrence the population, among which 1 percent is occupied by rheumatoid arthritis (RA). Remission this type disease extremely rare and requires constant use pharmacotherapy. Studying pathogenesis RA necessary to study search for new drug targets. It known that T helpers (Th) Th17 are involved development RA. However, some researchers suggest ILCs play role “innate analogues” Th, due fact subpopulation synthesizes same cytokines. ILC1 innate analogs Th1, ILC2-Th2, ILC3-Th17. tissue-resident lymphoid cells have functional diversity regulate direction immune response through production We used peripheral blood mononuclear (PBMCs) from patients (n = 19) conditionally healthy donors 10) as material. The group was divided biologic disease-modifying anti-rheumatic drugs (bDMARDs) Metotrexate (MTX) stage (early very early arthritis, advanced late). PBMCs were stained with monoclonal antibodies. identified Lin - CD127 + , CD294 (ILC2) measured general CD117-CD294-ILCs ILC1, CD117 ILC3. obtained following results: significantly reduced treated MTX comparison on bDMARDs donors. had low levels ILC2 ILC3 compared bDMARDs. increased stages MTX, Expression PD1 expression taking